MASCOT Search Results

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E-mail :  
Search title : 8plex iTRAQ from Inst. of Cancer Sciences, Univ. Manchester
Databases :

1: contaminants 20090624 (262 sequences; 133,770 residues)

2: SwissProt 2019_11 (561,568 sequences; 201,997,950 residues)
Taxonomy :

1: (none)

2: Homo sapiens (human) (20,368 sequences)
Timestamp : 9 Jan 2020 at 11:48:02 GMT
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Search parameters
Type of search : MS/MS Ion Search
Quantitation : iTRAQ 8plex (Applied Biosystems iTRAQ(TM) 8-plex)
Enzyme : Trypsin/P
Fixed modifications : Methylthio (C), iTRAQ8plex (N-term), iTRAQ8plex (K)
Variable modifications : Deamidated (NQ), Oxidation (M), Oxidation (HW), Acetyl (Protein N-term), iTRAQ8plex (Y)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm
Fragment mass tolerance : ± 0.3 Da
Max missed cleavages : 1
Instrument type : ESI-QUAD-TOF
Number of queries : 184,516

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event.

There are 36,368 peptide matches above identity threshold and 43,585 matches above homology threshold for 184,516 queries. On average, individual ions scores > 27 (beyond green shading) indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Protein score distribution. Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Modification statistics for all protein families
Modification Delta Type Site Total matches
iTRAQ8plex 304.20536 fixed N-term 43505
iTRAQ8plex 304.20536 fixed K 22023
Methylthio 45.987721 fixed C 11377
iTRAQ8plex 304.20536 variable Y 4662
Deamidated 0.984016 variable N 4469
Oxidation 15.994915 variable M 2854
Oxidation 15.994915 variable W 445
Deamidated 0.984016 variable Q 190
Oxidation 15.994915 variable H 167
Acetyl 42.010565 variable Protein N-term 33

Legend

Peptide columns and rows

Dupes … Expect Rank U 1 2 Peptide
  0.037 Expand2 GAYSLSLR significant
  9 Expand1 GFFLFVEGGR top ranking
  6.4e-05 Expand1 GSSIFGLAPGK significant and top ranking
  1.3e-06 Expand1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
  6.2e-07 Expand1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
  6.4e-05 Expand1 U GSSIFGLAPGK unique
Expand2   5.7e-05 Expand1 LNTLETEEWFFK peptide has two duplicates
  0.18 Expand1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (Expand) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (Collapse) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein quantitation ratios

Score … 114/113 115/113
CFAH_HUMAN 37559 0.962 1.129
FHR2_HUMAN 1330 0.859 1.128

When quantitation method is Reporter (e.g. iTRAQ) or Multiplex (e.g. IPTL), protein ratios are displayed when a family is expanded. Ratios in italic indicate that the peptide log-ratios do not appear to come from a normal distribution. Bold indicates that if you can assume peptide ratios are normally distributed, the protein ratio is significantly different from 1.0 (at significance level 0.05).

Note that lack of bold or italic can also mean that significance or normality testing has not been performed (for example, if protein ratio type does not support it).

Protein Family Summary

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Sensitivity and FDR (reversed protein sequences)

Target Decoy FDR
Protein family members 545 191 35.05%
36368 290 0.80%
Target Decoy FDR
Protein family members 545 191 35.05%
43585 600 1.38%
Target Decoy FDR
Protein family members 545 191 35.05%
2882 74 2.57%
Target Decoy FDR
Protein family members 545 191 35.05%
3310 200 6.04%

Decoy results are available in the decoy report.

Show

Protein families 1–10 (out of 521)

Page: 1 2 3 4 5 6 … 53 Next 

Expand1

Accession Score Description
Family member distances as a dendrogram 1 2::CO4B_HUMAN 164342 Complement C4-B OS=Homo sapiens OX=9606 GN=C4B PE=1 SV=2
2 2::CO4A_HUMAN 163856 Complement C4-A OS=Homo sapiens OX=9606 GN=C4A PE=1 SV=2

Expand2

Accession Score Description
1 2::APOB_HUMAN 127385 Apolipoprotein B-100 OS=Homo sapiens OX=9606 GN=APOB PE=1 SV=2

Expand3

Accession Score Description
1 2::CERU_HUMAN 59576 Ceruloplasmin OS=Homo sapiens OX=9606 GN=CP PE=1 SV=1

Expand4

Accession Score Description
1 2::A1BG_HUMAN 58870 Alpha-1B-glycoprotein OS=Homo sapiens OX=9606 GN=A1BG PE=1 SV=4

Expand5

Accession Score Description
1 2::HEMO_HUMAN 44576 Hemopexin OS=Homo sapiens OX=9606 GN=HPX PE=1 SV=2

Expand6

Accession Score Description
Family member distances as a dendrogram 1 2::CFAH_HUMAN 37520 Complement factor H OS=Homo sapiens OX=9606 GN=CFH PE=1 SV=4
2 2::FHR2_HUMAN 1329 Complement factor H-related protein 2 OS=Homo sapiens OX=9606 GN=CFHR2 PE=1 SV=1
3 2::FHR1_HUMAN 1289 Complement factor H-related protein 1 OS=Homo sapiens OX=9606 GN=CFHR1 PE=1 SV=2
4 2::FHR5_HUMAN 699 Complement factor H-related protein 5 OS=Homo sapiens OX=9606 GN=CFHR5 PE=1 SV=1

Expand7

Accession Score Description
1 2::ITIH2_HUMAN 35418 Inter-alpha-trypsin inhibitor heavy chain H2 OS=Homo sapiens OX=9606 GN=ITIH2 PE=1 SV=2

Expand8

Accession Score Description
1 2::FETUA_HUMAN 35203 Alpha-2-HS-glycoprotein OS=Homo sapiens OX=9606 GN=AHSG PE=1 SV=2

Expand9

Accession Score Description
Family member distances as a dendrogram 1 2::CFAB_HUMAN 31648 Complement factor B OS=Homo sapiens OX=9606 GN=CFB PE=1 SV=2
2 1::00718067.1 3489 (Bos taurus) Complement factor B

Expand10

Accession Score Description
1 2::APOH_HUMAN 30084 Beta-2-glycoprotein 1 OS=Homo sapiens OX=9606 GN=APOH PE=1 SV=3

Page: 1 2 3 4 5 6 … 53 Next 

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