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Welcome
Join us at the upcoming Matrix Science User Meeting on 3 June at ASMS 2024, Anaheim, California.
Are you presenting a poster or talk at ASMS? Did you use Mascot? Send us the poster/talk number, presenter name and abstract to newsletter@matrixscience.com and we will feature it right here.
In this month's highlighted publication, the authors show how wind can affect the proteins expressed in plants.
We are pleased to announce a new Mascot distributor in Europe.
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Mascot: The trusted reference standard for protein identification by mass spectrometry for 25 years
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Matrix Science ASMS User Meeting
Please join us at the upcoming ASMS User Meeting held on 3 June in Anaheim, California.
There is no charge for attending the meeting, but advance registration is required. Numbers will be limited, so early registration is advisable. Breakfast will be provided.
Monday 3 June, 7:00 am – 8:00 am
Room 213A, Anaheim Convention Center, Anaheim, CA
Preview of Mascot Server 2.9,
Ville Koskinen, Matrix Science Custom reporting with Mascot Distiller,
Patrick Emery, Matrix Science Automatically running reports with the Mascot Daemon Export Extender,
Richard Jacob, Matrix Science
Register now
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Featured publication using Mascot
Here we highlight a recent interesting and important publication that employs Mascot for protein identification, quantitation, or characterization. If you would like one of your papers highlighted here, please send us a PDF or a URL.
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The Quantitative Biotinylproteomics Studies Reveal a Wind-Related Kinase 1 (Raf-Like Kinase 36) Functioning as an Early Signaling Component in Wind- Induced Thigmomorphogenesis and Gravitropism
Nan Yang, Jia Ren, Shuaijian Dai, Kai Wang, Manhin Leung, Yinglin Lu, Yuxing An, Al Burlingame, Shouling Xu, Zhiyong Wang, Weichuan Yu, and Ning Li
Mol Cell Proteomics (2024) 23 100738
Wind is a common environmental factor that causes plants to develop various responses via a process called thigmomorphogenesis. In this study, the authors investigated how plants transduce wind force signals downstream.
After treating Arabidopsis plants to sustained wind of 4 m/s, they utilized two known mechanical signaling-related phosphoproteins – mitogen-activated protein kinase kinase 1 (MKK1) and 2 (MKK2) – as the baits. They then performed in vivo proximity labeling to identify putative protein-protein interactors of MKK1 and MKK2, tagging neighboring proteins within a distance of 10 to 20 nm.
A tandem enrichment protocol was used with both biotinylprotein and biotinylpeptide isolation steps to reduce the chance of false positives. After LC-MS/MS of the digests, quantification was performed using intensity-based absolute quantification as well as stable isotope labeling in the Arabidopsis.
In the MKK1 arm, 178 biotinylproteins were quantified with 12 found to be MKK1-associated proteins. In the MKK2 arm, 333 biotinylproteins were quantified with 72 found to be MKK2-associated proteins. Protein WIRK1 was found to be a common interactor of both MKK1 and MKK2, and involved in the wind-triggered response.
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New European Distributor for Mascot
We are pleased to announce a new distributor for Matrix Science products!
Customers in Europe can now buy Mascot through MS Wil B.V., a one-stop-shop for fine mass spectrometry tools. MS Wil is selling new licences for Mascot Server and Mascot Distiller as well as updates and upgrades to existing licences.
The new distribution agreement means buying Mascot has never been easier: specify Mascot as part of your instrument package when buying through Bruker, Shimadzu, Thermo or Waters; order from MS Wil when stocking up on lab consumables; or buy direct from Matrix Science Ltd.
If you have an existing support contract, there is no change to your support status. Licences bought through European distributors and marketing partners continue to be supported directly by Mascot developers based in the UK.
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About Matrix Science
Matrix Science is a provider of bioinformatics tools to proteomics researchers and scientists, enabling the rapid, confident identification and quantitation of proteins. Mascot continues to be cited by over 2000 publications every year. Our software products fully support data from mass spectrometry instruments made by Agilent, Bruker, Sciex, Shimadzu, Thermo Scientific, and Waters.
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You can also contact us or one of our marketing partners for more information on how you can power your proteomics with Mascot.
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