Mascot: The trusted reference standard for protein identification by mass spectrometry for 25 years

SCIEX Data Explorer

SCIEX Data Explorer is used to view and process data files from SCIEX Voyager instruments. Versions 4.2 and later also support the 4×00 and 5×00 series TOF/TOF instruments by opening the *.T2D files exported from the Oracle database.

Mascot Distiller

Mascot Distiller supports MS/MS data saved in T2D format. When creating a new project, simply choose SCIEX Data Explorer as the file format and browse to the *.T2D file.

T2D file open in Mascot Distiller

Mascot Daemon

Mascot Daemon can use Mascot Distiller as a data import filter, eliminating the need to create peak lists manually. Step one is to create a suitable parameter set and save it. If the parameter set is for searching MS/MS data, the file format should be specified as Mascot Generic.

In Mascot Daemon, choose ‘Mascot Distiller’ from the list of data import filters. This will be listed if Mascot Distiller is installed and registered on the PC running Mascot Daemon. Clicking on the Options button will invoke the dialog shown below. In most cases, you only need to specify the file format as ‘Sciex Data Explorer’ and choose a file containing suitable set of processing options. More details can be found in the Mascot Daemon and Mascot Distiller on-line help files.

Data Explorer 8

Mascot Daemon tasks can be batch tasks, where a list of files is searched either immediately or at some pre-set time, or can be real-time monitor tasks. These monitor a specified path for new data files. The advantage of a real-time monitor task is that data files can be submitted to Mascot immediately after acquisition and without any user intervention.

Data Explorer 7