Blog
Articles tagged: iodoacetamide
How much cashmere is in this sweater?
Textiles made from luxurious animal hair like cashmere and angora can fetch a high price. Bloomberg reported that cashmere garments alone were worth $1.4 billion in global exports in 2016. Big money attracts fraudsters, and sometimes products claiming to be cashmere could be something else, like rat fur. In this context, the International Organization for Standardization (ISO) has recently standardised [...]
Higher accuracy for oxidation profiles
Earlier this year, Reest et al. described a quantitation workflow based on light and heavy iodoacetamide labels, which they named SICyLIA [1]. The workflow was used for analysing cysteine oxidation levels between wild-type and redox-stressed mouse cells. At its core, SICyLIA is a precursor protocol with two components, light and heavy, where the labels are simply light and heavy carbamidomethyl on [...]
Step away from the iodoacetamide
In our July newsletter, we featured a paper from Torsten Müller and Dominic Winter, University of Bonn, concerning alkylation artefacts. Some of their findings were quite shocking. For example, differences of more than 9 fold in numbers of identified methionine-containing peptides for in-gel digested samples between iodine- and non-iodine-containing alkylation reagents. This is important because a glance at the literature [...]