Exercise MSMS4
Topic
iTRAQ quantitation
Requirements
User access to any Mascot server, including the Matrix Science public web site
Experimental
Data from an ESI quadrupole TOF instrument. Cysteine unmodified. Search Swiss-Prot with precursor and fragment tolerances of 0.2 Da, Trypsin/P with 2 missed cleavages, and iTRAQ 4plex quantitation method.
Data
Right-click this link: MSMS4.mgf, and choose Save target as or Save link as
Tips
- The iTRAQ modifications are specified inside the quantitation method. You don’t need to select them in the search form
Questions
- Are all four reporter ions being used?
- Why are some ratios shown in bold face?
- Would normalisation be a good idea for this example?
- How is iTRAQ modification to Tyr used in the method?
Hints | Search results | Answers