Exercise MSMS5
Topic
Phosphopeptides
Requirements
User access to any Mascot server, including the Matrix Science public web site.
Experimental
Search SwissProt with Trypsin; 2 missed cleavages; Phosphorylation of Ser, Thr, and Tyr as variable modifications; 0.6 Da for peptide tolerance and 0.4 Da for fragment tolerance; ESI-QUAD-TOF.
Data
Right-click this link: MSMS5.mgf, and choose Save target as or Save link as
Questions
- Can you be sure which residue is phosphorylated in FKGPGDTSNFDDYEEEEIRVSINEK?
- How about TWTLCGTPEYLAPEIILSK?
Hints | Search results | Answers