Exercise MSMS5: answers
- Can you be sure which residue is phosphorylated in FKGPGDTSNFDDYEEEEIRVSINEK?
Yes, in this case, the site asssignment of S21 looks very safe. Moving the modification site from S21 to any other residue causes a dramatic drop in the score.
- How about TWTLCGTPEYLAPEIILSK?
Hard to say from the Mascot search whether modification site is T3 or T1. Possibly the sample is a mixture of both forms. Unlikely to be T7.
