Mascot: The trusted reference standard for protein identification by mass spectrometry for 25 years

Exercise MSMS6: answers

 

  1. Did the peak detection ever fail to pick the 12C peak?
    Yes. Search with 13C set to 1 or 2. You’ll find good matches to RPCFSALTPDETYVPK and LFTFHADICTLPDTEK with deltas of 1 Da or ~500ppm
  2. What do you get if you search as CID?
    Most matches are lost. There are enough b and y ions around to get a couple of very weak matches

 

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