Exercise PMF1
Topic
A PMF that gives a very strong match. Treat it as a standard and use it to find optimum settings for the search parameters
Requirements
User access to any Mascot server, including the Matrix Science public web site
Experimental
A protein mixture (not alkylated) was separated on a 2D gel. A spot was extracted and digested with trypsin. The digest was analysed on a reflector MALDI-TOF instrument. The data file contains a list of MH+ values
Data
Right-click this link: PMF1.txt, and choose Save target as or Save link asTips
- The protein sequence is in SwissProt
- A very strong match
Questions
- What is a suitable mass tolerance?
- What is the optimum setting for missed cleavages?
- Is there evidence for Met-Ox?