Exercise PMF2
Topic
A more difficult PMF search. Learn to expect the unexpected!
Requirements
User access to any Mascot server, including the Matrix Science public web site
Experimental
A protein (not alkylated) from a 2D gel spot, digested with trypsin, and analysed on a MALDI-TOF instrument. The instrument is supposed to be capable of 25ppm accuracy, but it has not been used for a long time and the person who ran the spectrum is a novice. The gel was run as part of a study of baker’s yeast, (Saccharomyces Cerevisiae). The data file contains a list of MH+ values.
Data
Right-click this link: PMF2.txt, and choose Save target as or Save link as
Tips
- Take the settings from the first exercise (PMF1) as a starting point
- The protein sequence is in SwissProt
- A strong match
Questions
- What can we learn from this result?
- What advice would you give to the person who ran the spectrum?