Exercise SQ1
Topic
A simple sequence tag search
Requirements
User access to any Mascot server, including the Matrix Science public web site
Experimental
The sample was digested with trypsin and analysed on a ESI-QUAD-TOF. A tag has been interpreted from one of the MS/MS spectra. Precursor m/z is 738.25 with charge 2+. Residue code i means I or L and q means Q or K. Search Swiss-Prot with precursor and fragment mass tolerances of 0.5 Da
Tips
- Although the screen shot comes from Distiller, you should not submit your searches from Distiller. The aim of this exercise is to understand how to use the sequence tag syntax and web-browser search form
- You should be able to get a strong match using a standard sequence tag
Questions
- What is the peptide sequence?